Immunomodulatory effects of heat-killed Enterococcus faecalis TH10 on murine macrophage cells.
Case：Allergy / Immunity
Immunomodulatory effects of heat-killed Enterococcus faecalis TH10 on murine macrophage cells
MicrobiologyOpen. 1, 373-380 (2012)
The results of our collaborative research with the
Department of Fisheries, Faculty of Agriculture at Kinki University have been
published in the MicrobiologyOpen.
examined the immunomodulatory effect of heat-killed Enterococcus faecalis TH10
(hk-TH10) on murine macrophage RAW264 cells.
measured the amount of nitric oxide (NO) produced from a cell in order to
examine the macrophage activity which is an index of activation of the innate
immune system. We utilized Toll-like receptors (TLRs) which were knocked down
by small interfering RNA (siRNA) in order to determine the pathway in cells
where hk-TH10 stimulates macrophage activity.
we set lipopolysaccharides (LPS) which are known to be an enhancer of NO
production in cells as a positive control, hk-TH10 stimulated NO production up
to a level that is almost half of what LPS does. LPS is a strong enhancer.
Therefore, we consider that hk-TH10 has a moderate macrophage activation
According to the result from
the experiment using siRNA, macrophage RAW264 cells recognized the passage of
hk-TH10 through TLR-2 and TLR-6 pathways, and hk-TH10 stimulated NO production
via the activation of NF-kB.
Identification of 3-phenyllactic acid as a possible antibacterial substance produced by Enterococcus faecalis TH10.
Phenyllactic acid produced by Enterococcus faecalis TH10 shows high antibacterial effect against various kinds of disease-causing bacteria
Biocontrol Science. 9, 77-81 (2004)
The results of a collaborative research with the faculty of
Agriculture at Okayama University have been published in the Biocontrol
The culture fluid of E. faecalis TH10 isolated from tempeh
showed a high antibacterial activity against various kinds of disease-causing
germs. In this test, we tried to identify and refine the substance which
contributed to the antibacterial activity.
We extract culture supernatant of E.faecalis TH10 with
solvents and examined its fractions which has the antibacterial activity by reversed phase high-performance liquid chromatography (reversed phase HPLC). Subsequently, we performed gas
chromatography-mass spectrometry (GC/MS ) to identify an antibacterial agent.
on the test about fractionating culture supernatant by HPLC, a fraction eluted
by 80% methanol showed a high antibacterial activity against MRSA and the Eschericha coli O-157. As a result of
the identification test, E.faecalis TH10 produces phenyllactic acid and the
ratio of D- and L-rotatory phenyllactic acid was 2:1. The lactic acid bacteria
which produce D- and L-rotatory at the same time have not been found before, so
we found this is the original characteristic of E. faecalis TH10 and it
suggested that E. faecalis TH10 contributed to high antibiotic action.
Effects of fermentation product of herbs by lactic acid bacteria against phytopathogenic filamentous fungi and the growth of host plants.
The herb products fermented with lactic acid bacteria strongly inhibited the growth of phytopathogenic filamentous fungi
J Biosci Bioenginer 98, 187-192 (2004)
We examined the herb products fermented with lactic acid
bacteria in order to understand their inhibitory activity against
phytopathogenic filamentous fungi and their effect to the growth of host
We performed an antibacterial activity test on the lactic
fermented herb products such as Rosellinia necatrix, Helicobasidium
mompa, Fusarium oxysporum, Pythium graminicola and Pyricularia oryzae. We also germinated
seeds of alfalfa, asparagus, Komatsuna (Japanese mustard
rice, spinach, tall fescue and tomato on the plates containing 0.69 mg/ml of
the dried lactic fermented herb products.
lactic fermented herb products inhibited almost 100% the growth of
phytopathogenic filamentous fungi like R. necatrix, H. mompa, P. graminicola and P. oryzae. They inhibited 65% of
the growth of F. oxysporum. While they inhibited the growth of the phytopathogenic
filamentous fungi, they did not affect to the germinating rate of the seeds of