Publication



Complete sequence analysis of two cryptic plasmids from Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from healthy infant feces

Date 2014/01/01

Case:Identification of LAB

Determination of complete sequence of plasmids contained in Bifidobacterium kashiwanohense JCM 1543

Anim Sci J. 85, 158-163 (2014)


The results of a collaborative research with School of Veterinary Medicine, Azabu University have been published in the Animal Science Journal.

 

Objective

We analyzed 2 plasmids contained in bifidobacterium which were isolated from a healthy infant.

 

Methods

We cultured Bifidobacterium kashiwanohense JCM 15439 isolated from a healthy infant and purified plasmids pBBKW-1 and pBBKW-2. Subsequently, we determined and analyzed a complete sequence of these plasmids. We also performed an analysis with Southern-hybridization.

 

Results

The replicated plasmid pBBKW-1 was a rolling circle-type and replicated pBBKW-2 was a θ-type which has a better stability than a rolling circle-type.    

Anim Sci J. 85, 158-163 (2014)


The results of a joint research with School of Veterinary Medicine, Azabu University have been published in the Animal Science Journal. The research results show discovery of a new strain of bifidobacteria from healthy infants and information of plasmids this bifidobacteria holds.




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